Multiplexing of receptor occupancy measurements for pharmacodynamic biomarker assessment of biopharmaceuticals
I Vainshtein, AK Schneider, B Sun… - Cytometry Part B …, 2016 - Wiley Online Library
Background Receptor occupancy (RO) assays measure drug target engagement, and are
used as pharmacodynamic (PD) biomarkers. RO assays are commonly performed by flow
cytometry and often require multiplexing for assessment of multiple PD biomarkers when
specimen volumes are limited. We present multiplexed RO assays for an IGF1R‐EGFR
bispecific antibody (Bs‐Ab) and a CTLA4‐Ig recombinant fusion protein to demonstrate key
considerations for accurate RO assessment. Methods RO in cynomolgus monkeys was …
used as pharmacodynamic (PD) biomarkers. RO assays are commonly performed by flow
cytometry and often require multiplexing for assessment of multiple PD biomarkers when
specimen volumes are limited. We present multiplexed RO assays for an IGF1R‐EGFR
bispecific antibody (Bs‐Ab) and a CTLA4‐Ig recombinant fusion protein to demonstrate key
considerations for accurate RO assessment. Methods RO in cynomolgus monkeys was …
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